FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319268219
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT13109230.4106024095057203No Hit
AGGTCCGTCTTGCGCCGGTCCAAGAA10814150.3387167703027779No Hit
GTCTTCGAACCTCCGACTTTCGTTCT5192160.16262689773077602No Hit
GAGTCCGTCTTGCGCCGGTCCAAGAA4406810.13802845813475723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAG18734250.019.9072931
TAAGAGC18751700.019.7864742
AAGAGCA18752950.019.75373
AGGCCAC10146650.019.687351
TTTGCGC15370150.019.6828371
AACTCTT38072450.019.6728821
AGAGCAA19280250.019.6598244
ATGCAAC35061150.019.6345810
AGCAACG18853200.019.6267286
GAGCAAC18878800.019.6249565
TCTTTCA34848750.019.6071594
ATGTGAA11303350.019.603666
ACGCACC18953800.019.5996410
CTCTTTC35039800.019.5994723
TTTCATG34809950.019.5974226
ACTCTTT35815350.019.5894412
TTCATGC35076450.019.5746677
TCATGCA35425500.019.5710188
TGTGAAA11340550.019.5672177
AACGCAC18975300.019.567159
Produced by FastQC (version 0.11.5)