FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319268219
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13263950.41544849160197805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3278050.061.301499
GGTATCA3358500.059.2085348
AAGCAGT3685750.057.732651
TGGTATC3355550.057.1307147
GTGGTAT3511750.054.781086
AGCAGTG4395450.048.6663632
CAGTGGT4518800.043.607494
AGTGGTA4845100.040.170755
ATCAACG3455000.035.8975310-11
GCAGTGG6170550.034.8042563
TCAACGC3607150.033.47364412-13
CAACGCA3956250.033.2067912-13
ACGCAGA3856500.032.6407714-15
AGAGTAC3378950.031.43795418-19
AACGCAG4143400.029.63297314-15
CGCAGAG4115350.029.08765216-17
GCAGAGT3936550.027.73016416-17
TATCAAC3821650.027.62532410-11
CAGAGTA3730700.027.17229518-19
GTACTTT3031450.025.6809222-23
Produced by FastQC (version 0.11.5)