FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342796697
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGA1689450.018.7951771
GAATGAA1356250.018.5766181
CTCGAGG2261300.018.3207191
AGAAACG1719050.018.3106218
GACTAAC1203250.018.2872541
AAACGAG1213650.018.03710710
GTGATAA1188100.017.9680088
CCACTAC2148450.017.965771
AACTAGA1034000.017.9358272
GAGAAAC1413950.017.923737
CAACTAG1804100.017.8866141
CCACCTA1920350.017.7930221
AATGAAC914000.017.757812
CAATGGA1557800.017.7332618
CACCGAA818900.017.6398338
CAGACTA1085550.017.6349777
TCGAGGA741700.017.6139092
TGAGGGA1978950.017.5752681
ACCCGAG1112000.017.56953810
ACGGAGA633750.017.5687871
Produced by FastQC (version 0.11.5)