FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342796697
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5116690.14926310681459104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1687000.043.6373949
GGTATCA2023100.036.226828
AAGCAGT2451550.033.4733621
GTGGTAT2217650.032.1921546
TGGTATC2232400.031.7825437
ATCAACG1658100.029.9327610-11
CAACGCA1859700.028.25566112-13
TCAACGC1799550.025.91565112-13
AGCAGTG3263750.025.33232
ACGCAGA2077400.023.87190814-15
AACGCAG2217050.021.73221414-15
CAGTGGT3423850.021.5405084
CTACACG302600.021.3895971
AGAGTAC1867450.020.62651618-19
ACGACGC315950.019.8103075
AGTGGTA4003800.018.5764055
CGCAGAG2432200.018.48379916-17
TATCAAC2122200.018.28359610-11
CAGAGTA2197000.016.5228518-19
GCAGTGG5173900.016.0870253
Produced by FastQC (version 0.11.5)