FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358574979
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4093680.11416524408413896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1563250.040.6869059
GGTATCA1891750.033.2708058
TGGTATC1860950.032.3506777
GTGGTAT2091150.029.185286
AAGCAGT2440100.029.170661
ATCAACG1509800.028.98332610-11
CAACGCA1727450.026.9731912-13
TCAACGC1689550.024.49774612-13
AGCAGTG3118850.023.1081562
ACGCAGA1904000.023.10239414-15
AACGCAG2116000.020.251314-15
CAGTGGT3283000.019.2571934
AGAGTAC1788900.018.68464318-19
ACGACGC306300.018.5621345
CGCAGAG2246000.017.76806616-17
TATCAAC2004900.016.89665810-11
AGTGGTA3803600.016.8037455
CTACACG348950.016.5390381
CAGAGTA2106050.015.05808818-19
GCAGTGG4823650.015.0353163
Produced by FastQC (version 0.11.5)