FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353472372
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3899020.11030621652093364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1527650.040.6289949
GGTATCA1844050.033.2786188
TGGTATC1878550.031.2367237
GTGGTAT2004200.029.8045276
AAGCAGT2523450.028.2599031
ATCAACG1515350.028.23315410-11
CAACGCA1691600.026.8481312-13
TCAACGC1601550.025.24052212-13
AGCAGTG3200800.022.5695782
ACGCAGA1909600.022.44088714-15
AACGCAG2048650.020.60601414-15
CAGTGGT3207000.019.3336854
AGAGTAC1680000.019.24769818-19
ACGACGC280850.018.5642345
CGCAGAG2266300.017.27812416-17
TATCAAC2013700.016.43892510-11
CTACACG324150.016.3652761
AGTGGTA3942450.016.1505995
GCAGTGG4698650.015.3679113
CAGAGTA2073500.014.844398518-19
Produced by FastQC (version 0.11.5)