FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338134451
Sequences flagged as poor quality0
Sequence length8-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTTGGTTGATTGTAGATATTGGGC3972990.11749734427386105No Hit
ACGCCAGAGTCTCGTTCGTTATCGG3601420.10650852018624982No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT3570070.10558137419721246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGAG1451050.018.1837025
TACTAGA1231250.018.0926674
ATGGAGA1268250.018.0910534
GGAAAGC1517250.018.0877421
GATGAAA1017950.017.9386711
CCCAAAC601550.017.90183620
GAAATGA1399250.017.86261
TGACTAG1110600.017.8153271
GAGAAAC1100150.017.7619977
AGACTAT1491000.017.75473210
GCGCGAT1022850.017.715871
CATGGAG1141100.017.707283
CGGCTAG1348500.017.6741491
GCACTCT2647500.017.5587061
TAGTTGG1936600.017.5260261
AATACAG1081250.017.43458710
CCGGAAA740550.017.3983210
ACAAGAG1322050.017.3840873
CTACATT2445650.017.3681891
GTATGAA1073900.017.3292488
Produced by FastQC (version 0.11.5)