FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338134451
Sequences flagged as poor quality0
Sequence length8-98
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA8396440.24831660823581683No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT7995540.2364603777093391No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT7096850.20988248843061544No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG6896260.2039502328025132No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT6643890.19648663365567565No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA6635260.19623140973588638No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC6410600.1895873070916397No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT5752460.17012345186915012No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT5735840.169631931411804No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA5641520.16684250845531265No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG5334330.15775766072413602No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5056990.14955559792988973No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA4849340.143414549616537No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC4724960.13973613117582037No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT4648610.13747815362357146No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA4606180.13622332732963668No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG4392830.12991370701827717No Hit
GGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACG4376470.12942987580996296No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC4345700.12851988276107365No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT4165530.12319152892232207No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA4013680.11870071174735165No Hit
CTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTT3980200.11771057306432227No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG3920260.11593790542212452No Hit
GCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG3901360.1153789561655757No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC3838080.11350751124735291No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT3680130.10883629246048046No Hit
CCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTA3596140.106352369282833No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG3590450.10618409302517358No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTG3582400.10594602204553241No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT3563380.10538352390481501No Hit
GGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCT3489670.10320362180427453No Hit
GCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATT3469240.1025994242745765No Hit
CACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAG3404870.10069574365848928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1539150.056.084459
GGTATCA1745250.048.6920288
TGGTATC1790900.045.6744167
AAGCAGT2246500.044.8833351
GTGGTAT1924650.043.2209246
AGCAGTG2860100.035.0136642
ATCAACG1774900.033.51535410-11
CAACGCA1966500.031.36237512-13
TCAACGC1929750.029.162912-13
CAGTGGT2949100.028.7262334
ACGCAGA2046450.028.64668814-15
AGTGGTA3121600.027.1640685
CTACACG238100.027.1043761
AGAGTAC1703650.025.81788818-19
GCAGTGG3949700.024.661363
ACGACGC268400.023.6422255
AACGCAG2463800.023.60742214-15
CGCAGAG2291300.023.55156316-17
CAGAGTA1930600.021.7917118-19
TACACGA305350.020.7687472
Produced by FastQC (version 0.11.5)