FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317206073
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA779450.017.961141
GAACCTA1212450.017.619511
CAATGGA1063150.017.4809998
GATGAAA579050.017.4568581
TGCCAAA1181900.017.3769871
GGAAACA611700.017.1970710
GAAATGA863650.017.1800671
CCTCTGA1615900.017.136211
ATTGCGA678550.017.11142710
CGGACTG1033100.017.05121
GACTAAC731800.017.0300711
ACTGATG939550.017.017181
TACGGAT979600.016.9124261
ATCAACA894350.016.9110667
ACTTGAA658100.016.89387510
CAACTAG1248950.016.868871
AAACCTG1015700.016.8560091
GCAGTTA907300.016.8239251
ATGCGAT805050.016.8218151
CCGGGAT793150.016.8176141
Produced by FastQC (version 0.11.5)