FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376851731
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT3901770.10353594475064254No Hit
AGTTGGTTGATTGTAGATATTGGGCT3815430.1012448580208326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCA3437450.018.643051
ATAAGAG1567150.018.4534441
TGCGAGA1630050.018.4146524
CGAGCAC1893600.018.319661
TAAGAAA883150.018.2748556
ATAGACC1790900.018.258161
CAATGGA2270450.018.1587528
CCGGAAA1116150.018.11674710
ATAAGAA907600.018.1162855
GGTATTG2936600.018.1122151
ACTAGCA1573800.018.1003533
TGACTAG1788950.018.0390321
TTGAAAG1098700.017.9879683
GAACCTA2902250.017.9043521
GCGAGAT893800.017.900865
GAAATGA1565950.017.889841
CGAACAT2376000.017.8755341
GGAACTT1743450.017.8392961
GACTAGC1465400.017.8328482
CGGCCAA1122700.017.8162442
Produced by FastQC (version 0.11.5)