FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005798567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376851731
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT4984790.13227456821738734No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT4204010.11155607508672952No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG4135890.1097484676274447No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC4105190.10893382363155445No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT3855350.1023041605718404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1198750.040.741929
AAGCAGT1763450.033.842411
GGTATCA1445400.033.2363328
TGGTATC1586900.029.2486067
CAACGCA1260300.028.65311612-13
ATCAACG1265850.027.12894410-11
GTGGTAT1803000.026.4466936
TCAACGC1193600.026.43655412-13
ACGCAGA1588850.022.41767914-15
AGCAGTG2950900.020.2919032
ACGACGC262600.019.596015
AGAGTAC1354350.019.07863218-19
AACGCAG1908600.018.10798514-15
CGCAGAG1782750.017.85707916-17
CTACACG310400.017.583871
CAGAGTA1610550.015.56754218-19
GCAGTGG3731200.015.0358483
AGTGGTA3741600.013.6235395
ACACGAC391700.013.5542833
GTACTTT1500700.013.44903722-23
Produced by FastQC (version 0.11.5)