FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374474064
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT3774620.10079790198767945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1050600.041.5232249
AAGCAGT1651800.032.1917421
CAACGCA1064300.029.66451812-13
GTGGTAT1464400.029.4109176
ATCAACG1044500.028.76708610-11
TCAACGC1074050.026.32443612-13
GGTATCA1682900.026.0859838
ACGCAGA1458100.021.67836214-15
AGCAGTG2506050.021.4420682
TGGTATC2016600.021.4402077
AGAGTAC1132750.019.9965618-19
ACGACGC225150.019.7002285
AACGCAG1567900.019.57333214-15
CGCAGAG1618600.017.74147416-17
GCAGTGG3071700.016.3605523
CTACACG318850.014.4670971
AGTGGTA3189100.014.3188365
CAGTGGT3131650.014.2771494
CAGAGTA1611750.013.96425218-19
GAGTACT1611600.012.59038620-21
Produced by FastQC (version 0.11.5)