FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379936337
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC5597720.14733310438795963No Hit
CGTTCGTTATCGGAATTAACCAGAC4690920.12346594792800773No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT4332380.11402910377587812No Hit
TTCGGTCTTGATTAATGAAAACATTC4251590.11190269489806658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAAA841950.018.1222349
CACACAA1832800.017.5156061
CGAGAAG864400.017.5083261
GAACCTA1503450.017.476831
CGCGAAA962100.017.45826310
CTGCGGA1588650.017.4172611
ATAGACC1211950.017.414281
ACTGAAC936100.017.3998241
TCTGGAA1280250.017.3525681
TCCACGA1362450.017.3332828
GTATGAA1106600.017.2990158
CAATGGA1509050.017.2515838
GGGAATG1143750.017.2378351
TATTGAG869650.017.1746543
GGGACCT1176350.017.0912551
TACACGA803350.017.0331651
AGCGAGA754200.017.0210278
AATGGAA742050.017.001049
CTGCCTA1323300.016.9810581
CGCTTCA1502750.016.938341
Produced by FastQC (version 0.11.5)