FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379936337
Sequences flagged as poor quality0
Sequence length8-98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA844650.034.981789
ATCAACG802550.025.31339610-11
GGTATCA1218550.023.8835538
AAGCAGT1600700.021.995411
CAACGCA969450.021.99012412-13
GTGGTAT1380800.020.5760976
TCAACGC935600.020.27300812-13
ACGCAGA1215500.017.87980714-15
TGGTATC1639800.017.2921477
AGAGTAC984900.016.87209518-19
AACGCAG1272850.016.41965114-15
AGTGGTA2096000.014.4870835
AGCAGTG2453250.014.0967772
CGCAGAG1431150.013.95653616-17
ACGACGC282000.013.8126575
CAGTGGT2194000.013.3090184
CACGACG343900.011.3743914
TATCAAC1575650.010.68942210-11
GAGTACT1522550.09.98698920-21
GCAGTGG3448750.09.6398353
Produced by FastQC (version 0.11.5)