FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369206717
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1134450.046.9596189
GGTATCA1533550.034.6490758
GTGGTAT1507400.034.0491146
AAGCAGT1877300.033.2835771
CAACGCA1296850.030.73141912-13
ATCAACG1260000.030.18956610-11
TGGTATC1729600.029.4892277
TCAACGC1289550.027.89187412-13
ACGCAGA1588350.025.4139714-15
AGCAGTG2794300.022.6527732
AGAGTAC1296200.022.29939518-19
ACGACGC239900.020.1899535
CGCAGAG1913200.019.53304716-17
CAGTGGT2775850.019.0383434
GCAGTGG3320950.017.9311313
AACGCAG2293250.017.3636714-15
AGTGGTA3262850.016.7163095
CAGAGTA1790000.016.00069818-19
GCAGAGT1997000.015.60384716-17
CACGACG342700.014.2548834
Produced by FastQC (version 0.11.5)