FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354357095
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1365700.017.8009471
GTCGTAA1454350.017.3471011
CAATGGA1440000.017.3321088
CCGGGAT1089400.017.2279741
TGGCTGG1589450.017.217991
ATTGGTG1373050.017.0385111
GATCTAG1276200.017.0284061
GAATGAA1136800.016.9929391
CGATTGA1146300.016.8736611
GACTAAC959750.016.8597531
ACCCACT1326500.016.8356231
GACCAAT1294500.016.7983671
TGAGCAT1268650.016.7434061
CTTAACT1204000.016.6928751
CGCTTCA1059350.016.6775321
ACTGAAC883300.016.595031
CGTTGGG1696200.016.5887891
ACGAGGA945000.016.537331
ACGGAGA1144950.016.5094951
TATGAAA686800.016.500349
Produced by FastQC (version 0.11.5)