FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354357095
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1161350.029.1794839
ATCAACG894650.026.99653210-11
CAACGCA1113950.022.82149112-13
TCAACGC1102350.020.67430912-13
GTGGTAT1654900.019.8127336
AAGCAGT2088500.019.7979931
AACGCAG1260750.018.90273914-15
ACGACGC223350.018.2374695
GGTATCA1884900.018.1287168
AGAGTAC1073250.016.84836418-19
ACGCAGA1480800.016.51718914-15
TGGTATC1979500.016.4483017
AGCAGTG2588750.016.118182
AGTGGTA2238350.015.6216135
CTACACG296450.014.3109811
CGCAGAG1550150.014.30960416-17
TATCAAC1360300.013.55111110-11
CAGTGGT2676650.012.9102524
CACGACG316200.012.8699474
GCAGTGG3854750.010.6270183
Produced by FastQC (version 0.11.5)