FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395979110
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCAGAGTCTCGTTCGTTATCGG4276550.10799938410892435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC1454350.018.1945951
AAACCTG2061050.018.0217511
TGACGGC2078850.018.0129951
ACTGAAC1200400.017.8417591
AATAAGA634500.017.8397962
GGGAGAT1090600.017.7630021
TATGAAA753850.017.7545139
TTAAGAA818200.017.702699
AGGACGA929100.017.6145488
GGACGAA931750.017.5592299
GATGCTA1550200.017.4791281
GAATGAA931100.017.428661
TGGCCAG1552400.017.4205991
GAAATGA857650.017.4108871
GTATGAA1201950.017.3072728
GGGCATC1709500.017.2709751
GGCTCGA1339900.017.269061
ACGAGCC1877150.017.1960871
CCGGTAG1728250.017.1791521
ATAACGA644950.017.16331910
Produced by FastQC (version 0.11.5)