FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395979110
Sequences flagged as poor quality0
Sequence length8-98
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG231100.022.8485951
GTATCAA739550.019.1651769
ATCAACG470700.018.19991310-11
TCAACGC498600.016.22451612-13
ACGACGC333650.015.5306575
TACACGA371000.014.2769632
GGTATCA1121950.012.6826738
CAACGCA696550.012.64146512-13
TAGCGCG560250.010.25432192
AGAGTAC630950.09.71996518-19
AAGCAGT1664450.09.6523891
AGCAGTG1906500.08.4739342
ACACGAC645300.08.2507323
ACGCTCT675450.08.1530038
TCCGATC413250.07.720343614-15
CACGACG722150.07.42269234
AGTGGTA2134850.07.28173455
CGACGCT742850.07.1971876
CGCTACT486150.06.68592792
AACGCAG1338300.06.66137214-15
Produced by FastQC (version 0.11.5)