FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355904291
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT4717980.1325631671015734No Hit
ACCTTTAAGTTTCAGCTTTGCAACC4174990.11730653733534223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA1192950.018.2847371
GGCGACT1208350.017.8288541
ACGCCAG2282850.017.819091
GACTAAC1053700.017.5235651
CAACTAG1769450.017.4815351
CTAATAG1021600.017.3820173
GCGCGAT813550.017.299381
CCTCTGA2036000.017.2706261
CTTAGGA1715000.017.2526381
CGTTGGG1891900.017.1590731
ACTAGCA1230300.017.129473
AAGGCAG1427200.017.0358241
AGACGTT1676250.017.0124321
GGGACCT1152250.016.977651
GCAATGA845900.016.9704577
TAATAGA568350.016.924724
CGGACTG966650.016.9078671
CATCAAG1167800.016.9041751
GGGCATC1459100.016.8994941
AAGCCGC2044550.016.881241
Produced by FastQC (version 0.11.5)