FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355904291
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA7739020.21744666180492891No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT7313410.20548810972329637No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT7244250.20354489066837356No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC7047880.19802739607879583No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT7006140.19685460887011336No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA6893610.1936928037768446No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG6775630.1903778676273392No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA6298000.17695768663828781No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA5692150.15993485169865512No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA5568340.15645610746513872No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG5239330.1472117682334996No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT5167340.14518903341909975No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT5073260.14254562612171484No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT5059720.14216518676365156No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT5040230.14161756762859598No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC4909190.13793567889295272No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG4427920.12441322321680016No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT4224490.11869736069015252No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC4145170.11646867162947469No Hit
GCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATT4144140.11643973126471803No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG3982080.11188625989339365No Hit
GCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG3921140.11017400180769385No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC3881170.10905094707048643No Hit
GGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACG3752280.1054294678340925No Hit
GGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCT3692330.10374502621548892No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA3685170.10354384853426789No Hit
GCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG3682440.10346714251894197No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT3678800.10336486783184078No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT3620750.10173381135210871No Hit
AGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCT3580620.10060626102425946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1092800.047.5431489
GGTATCA1321700.039.0140238
TGGTATC1344200.036.8276757
GTGGTAT1548750.032.5025986
ATCAACG1096200.030.61088410-11
AAGCAGT1874600.030.3278331
CAACGCA1194350.028.95632412-13
TCAACGC1183150.026.94904312-13
ACGCAGA1437150.023.25159514-15
AGCAGTG2486900.022.961722
CTACACG261200.021.0416131
AGTGGTA2432700.020.8303995
CAGTGGT2509500.020.2606984
AACGCAG1637300.020.22885114-15
AGAGTAC1328650.019.85324918-19
ACGACGC282300.018.8747235
CGCAGAG1815250.017.2208316-17
CAGAGTA1519450.016.7079518-19
TACACGA336200.015.9562052
GCAGTGG3820100.014.8822023
Produced by FastQC (version 0.11.5)