FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389774919
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC4363700.11195435589327903No Hit
CCACCTACTAAGAACGGCCATGCACC3936240.10098751377073598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTTA3070200.018.73211
TGAAGAC2120950.018.4494635
GGACGAA1051700.018.354229
GGCCGAA1464000.018.314019
GAAGACA1346450.018.3084436
AAGCTAA1327600.018.2942497
GGAAACA1196550.018.20614410
AATAAGA916800.018.1950722
GCAGGAA1378500.018.142537
ACTAGAG1617950.018.1006433
AGGACGA1068550.018.0803858
CTAAACA1392350.018.07904210
GCAATCA2168400.018.0652351
CAATGGA2336800.018.0166158
GCCTCTA2938900.018.015471
GAAATGA2460750.017.9342751
AGAGTAA1627200.017.9032528
CTTAGGA1421700.017.901151
CTACCCA2376600.017.895371
AATGGAA1245900.017.8746059
Produced by FastQC (version 0.11.5)