FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389774919
Sequences flagged as poor quality0
Sequence length8-98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4304270.11042962977307424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1403550.041.766669
GGTATCA1652700.035.321628
AAGCAGT2278650.029.335431
ATCAACG1387050.026.50022310-11
TCAACGC1429900.024.69074212-13
AGCAGTG2719750.024.6407012
CAACGCA1561050.024.25403212-13
AGTGGTA2674850.022.3987485
CTACACG273600.021.7600231
GTGGTAT2765750.021.152896
TGGTATC2865100.020.2186477
AGAGTAC1502350.019.9178718-19
CAGTGGT3008700.019.8026854
ACGCAGA2046100.018.77252414-15
ACGACGC322400.018.4159815
AACGCAG2230750.016.93134714-15
GCAGTGG4167800.015.8014953
CGCAGAG2336700.015.46843716-17
TACACGA400600.014.89337352
GTACTTT2082650.013.09087122-23
Produced by FastQC (version 0.11.5)