FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360857897
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT3741550.10368485853033721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA1093550.017.9175781
CTCGAAA876200.017.7273161
GATGAAA837250.017.6001421
GAATGAA859500.017.4259221
TACGGAT1396100.017.3035031
CAATGAA683750.017.280088
GACTAAC857250.017.075051
AGAAAGG881950.017.0562810
TCGGAAA775500.016.996658
GGAATAA1695250.016.96841
CCTACCA1396700.016.9362551
TATTGAG742900.016.883943
GGGAATG844550.016.879951
GTATGAA798950.016.8650268
TCTGGAA984600.016.8618891
AATAACA687700.016.8435383
AGAATAG1650150.016.7798041
CCGGGAT833500.016.7552831
CGATTGA1147950.016.7438161
CCCTCCT2419300.016.7186371
Produced by FastQC (version 0.11.5)