FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360857897
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4677940.1296338541816642No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT4158450.11523788268377566No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG3758260.10414792169561415No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT3656870.10133822843843708No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT3645330.10101843496582812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1167650.046.7226229
GGTATCA1523200.035.9238328
GTGGTAT1582050.033.541796
AAGCAGT1925250.032.5238341
ATCAACG1193600.030.55077610-11
TGGTATC1767400.029.6513867
CAACGCA1331650.028.3805912-13
TCAACGC1338600.026.37335612-13
AGCAGTG2687950.023.7813282
ACGCAGA1558900.023.16612614-15
AGAGTAC1210550.021.94655218-19
ACGACGC239050.021.00195
AACGCAG1749750.020.94406514-15
GCAGTGG3096350.020.3193953
CAGTGGT2766650.020.0796834
CGCAGAG1712400.019.76555316-17
TATCAAC1761400.016.61553410-11
GCAGAGT1795550.016.01313816-17
AGTGGTA3713200.015.23389155
GTACTTT1566350.015.09758322-23
Produced by FastQC (version 0.11.5)