FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332631961
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC6249140.1878694994074848No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT4399240.13225548100592777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA2503650.018.8580191
CACACAA2163050.018.4466361
CAATGAA1311750.018.3111488
CCGGGAT1476850.018.2909181
GCCGAAG915250.018.1950873
AGAAAGA699500.018.1466834
GGGACCT1577200.018.0509531
CAGAAAG1605950.018.0434533
CATGGAG1578350.017.990723
TTGGAAC1256500.017.9748021
ATGGAGG1599250.017.931474
GAATAAG1277900.017.8204841
CCACTAC1736350.017.8039421
GGGAGAT856650.017.8037991
GTGATAT1642000.017.77525710
AGGCCAC2117150.017.7751431
TAAATGA923250.017.7168249
GTAATGA1559500.017.6688710
AAATGAA779850.017.6675222
CGCGAAA831850.017.6362110
Produced by FastQC (version 0.11.5)