FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332631961
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9633770.28962249962504355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2184850.054.3040289
GGTATCA2393000.049.3098758
AAGCAGT2723650.048.7008741
TGGTATC2450450.047.0850877
GTGGTAT2565850.045.3215076
AGCAGTG3555650.037.1538732
ATCAACG2302550.032.69994710-11
CAGTGGT3674950.032.2301564
CAACGCA2492550.031.30115712-13
TCAACGC2450150.028.83869712-13
ACGCAGA2644850.028.83841114-15
GCAGTGG4627000.027.876623
AGAGTAC2345050.027.20233218-19
AACGCAG2749700.026.43927614-15
AGTGGTA4853600.024.7751395
CGCAGAG2889150.024.16548216-17
CTACACG244200.023.2620071
CAGAGTA2653950.022.63441718-19
GTACTTT2581850.021.20168122-23
TATCAAC3037650.020.49653410-11
Produced by FastQC (version 0.11.5)