FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369466441
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCCTAATCATGGCCTCAGTTCCG4359710.11800016229349501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA7924400.019.8131541
CTGATAG4824300.019.4880081
CACACAA5797000.019.466321
GATGTAA5124150.019.3852522
GTTTCTA7857100.019.3769361
AACAAAC6092600.019.3548556
TAAGTGC5887550.019.3483771
AAGGGAT3713900.019.2662437
CCACGAA5761200.019.2536267
ACACAAC5776750.019.2397922
GATGCTA3683750.019.2314741
AGGTGAA5256950.019.2021772
GCCACGA5721650.019.1937146
ATCGTAC6548150.019.1731057
GGAAAGC2004950.019.1675221
AAGGTGC5258600.019.1295767
CGAAACG5852850.019.11660610
TGTAAGC5198450.019.1087154
ACGAAAC5860650.019.1046339
TGAGCAT3652650.019.0944581
Produced by FastQC (version 0.11.5)