FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369466441
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA976600.032.1209839
GGTATCA1105700.028.1564038
ATCAACG859450.025.17284210-11
CAACGCA900600.025.07475912-13
ACGACGC339700.024.4930135
TCAACGC891300.022.74830812-13
CTACACG377550.022.5377391
ACGCAGA1232150.018.66541114-15
TACACGA451950.018.5397572
AAGCAGT2107250.017.84511
AGAGTAC1089850.016.24007418-19
CAGTGGT2018500.015.6384654
CGCAGAG1364050.015.37971616-17
TGGTATC1979400.015.3216437
AGCAGTG2483200.014.9459452
ACACGAC605800.013.8490633
CACGACG602900.013.8388774
ACGCTCT657100.012.5778538
GCAGTGG3059200.011.6990993
GTGGTAT2709450.011.4682796
Produced by FastQC (version 0.11.5)