FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361604742
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGGCTGTTAATTGTCAGTTCAGTG5657950.15646780428559756No Hit
GTCTCGTTCGTTATCGGAATTAACCA3926870.10859564446751642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA1346950.018.7618541
GACTAAC1407150.018.7386261
GACCAAT1587700.018.3105751
AGTGAAC1341000.018.2068778
ATAGACC1246150.018.1948281
CTCGAAA906400.018.099421
ACTGATG1460950.018.0554711
AAGTGAA1128100.017.960817
GATGAAA855750.017.9361461
ACAGCCG2166000.017.8659971
TCTGGAA1180300.017.8152241
ACTGAAC968150.017.7925741
GTGCATA1654650.017.7086031
GTCGAAA689900.017.6174038
CAACTAG1716900.017.5943811
TAGAAAG859900.017.527429
GCCGAAG807050.017.4978053
AGAAAGG934650.017.39353210
CGTTGGG2784650.017.385491
ACGAGGA966050.017.371071
Produced by FastQC (version 0.11.5)