FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361604742
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4172420.11538620807135323No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA4076540.1127346941705759No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT3835750.10607576600862165No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA3741820.1034781783918088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1534150.047.6901559
GGTATCA1765450.041.251398
TGGTATC1920600.037.2341087
GTGGTAT2001100.036.2618066
AAGCAGT2420700.035.601191
ATCAACG1588100.030.26569710-11
CAACGCA1717450.029.28505312-13
AGCAGTG3125700.027.3587022
TCAACGC1663650.026.8496212-13
ACGCAGA2019650.024.7906114-15
CAGTGGT3019050.024.6955954
ACGACGC252450.023.7024755
GCAGTGG3661950.022.4755883
AGAGTAC1844750.020.92363218-19
CGCAGAG2197350.020.58305216-17
CTACACG304250.020.3285241
AACGCAG2431350.019.72745514-15
GTACTTT1765100.017.82244122-23
GCAGAGT2261600.017.80010816-17
CAGAGTA2286450.016.2701118-19
Produced by FastQC (version 0.11.5)