FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332335652
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA954950.018.0598471
ACTGAAC1112950.017.9399171
TCTGGAA1122000.017.542251
GATGAAA747750.017.4945181
ACTGATG1125700.017.3725341
CACCGAA764000.017.3070268
GTGATAA765100.017.2564148
AATCGAA695950.017.251329
GACTAAC892650.017.2414191
CGATTGA965200.017.0992221
GGGATGA984950.017.096541
GGCGACT805150.017.0098651
CTGATAG1031500.017.0062031
ATCTAAC959400.016.9222987
GGAATAA1309200.016.8977261
CTCGAAA512850.016.771851
CAATGGA896200.016.7429878
ACCGAAA666100.016.7078179
TTGTAAA625600.016.6779296
TAGGTGA872600.016.6620247
Produced by FastQC (version 0.11.5)