FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332335652
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6389670.19226555927860547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1906900.051.2821359
GGTATCA2196700.043.514928
TGGTATC2167500.041.9748957
AAGCAGT2718100.041.0314641
GTGGTAT2510800.036.335796
ATCAACG2045300.032.5920510-11
AGCAGTG3478650.031.5393092
CAACGCA2345650.030.29002612-13
TCAACGC2253700.027.5799112-13
CAGTGGT3649050.025.9442044
ACGCAGA2587350.025.88861814-15
AACGCAG2601550.024.50273514-15
AGAGTAC2117950.024.12723418-19
GCAGTGG4462650.023.5770763
TATCAAC2451950.021.7109310-11
CGCAGAG2875550.020.70852516-17
GCAGAGT2608600.020.06165716-17
CAGAGTA2470700.019.59780718-19
GTACTTT2107500.019.57234422-23
AGTGGTA5401850.017.8724785
Produced by FastQC (version 0.11.5)