FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365460158
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTGGTTGATTGTAGATATTGGGCT5711300.1562769531774788No Hit
CCTCTATATAAATGCGTAGGGGTTTT4596230.12576555609106918No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG3863040.10570345126376264No Hit
CGTTCGTTATCGGAATTAACCAGACA3704350.10136125426837911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAAA1399550.018.4782731
TGAGCCG1814400.018.355621
GATGAAA907800.018.2478371
ATAACGA993550.017.98392910
CAAGGCC1824800.017.8753191
CGAGCAC1393000.017.8490521
CGGACGT1287150.017.7982671
GTGATAA1092050.017.7210378
CAATAAC1015100.017.6281858
GAAATGA1205500.017.6109391
AGAGACG1608950.017.60756910
TGCGTGG1973950.017.59881
AAATGAG806900.017.5910022
GAATAAG974450.017.5841061
ATTGGAC1726650.017.5798381
GACCAAT1516100.017.5132711
ATCGAGT1089550.017.5094341
CGGACTG1012950.017.4699631
TTGTAGG1660350.017.432921
AATAACG1027250.017.4040539
Produced by FastQC (version 0.11.5)