FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365460158
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA4576070.12521392277184973No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT4243760.11612100271679957No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA4121490.11277535758083923No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC3987520.10910956810783187No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA3916220.10715860304531472No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG3906130.1068825127580665No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT3726690.10197253841279191No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT3656940.10006398563424251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1326250.043.3857469
GGTATCA1487350.038.3630078
TGGTATC1540300.035.919077
AAGCAGT2168000.030.7354471
GTGGTAT1914100.029.4199246
ATCAACG1351000.028.27572610-11
CAACGCA1471250.027.22754712-13
CTACACG321950.025.8641871
TCAACGC1508300.023.66670612-13
TACACGA368450.022.5189632
AGCAGTG2952400.022.356032
ACGCAGA1844550.021.69458614-15
CAGTGGT2825100.020.6332324
AGAGTAC1615150.018.98230418-19
CGCAGAG1942500.018.77059716-17
GCAGTGG3424700.018.671643
ACGACGC442550.018.451275
GTACTTT1535300.016.20889922-23
AACGCAG2461550.015.85972114-15
GCAGAGT2134650.015.06662716-17
Produced by FastQC (version 0.11.5)