FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329529400
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCC7709470.2339539355213829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC1157800.018.0354941
CTCGAAA924300.018.00741
TTGGCAA1542800.017.478061
CAATGGA1110000.017.418318
GAATAGC1116400.017.297322
ATAAGAA381900.017.2604085
TGCGAGA721800.017.2597164
CACCGAA682750.017.259428
GACTAAC829800.017.2505911
GGAATAA1040550.017.2487261
TGACTAG921250.017.1184331
GACCAAT1052300.017.1123661
GCGCGAT837550.017.0379731
AATACAG843250.017.03138410
GTATGAA918050.016.952818
GACCTGG1524100.016.835031
GGGAATG801700.016.8021031
TGGCCAG1282900.016.7519251
GGGAGAT754250.016.7488961
TCGGAAA705800.016.7180758
Produced by FastQC (version 0.11.5)