FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329529400
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC7884970.23927971221991118No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6836270.20745554114443202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2014700.050.1749539
GGTATCA2289250.043.3769728
TGGTATC2263750.042.1560977
AAGCAGT2718500.041.6855351
GTGGTAT2591550.037.350686
ATCAACG2069900.031.3024710-11
AGCAGTG3613900.031.2665272
CAACGCA2270900.030.34278112-13
CAGTGGT3471300.028.4595074
AGTGGTA3547800.028.236655
TCAACGC2155600.027.96567312-13
ACGCAGA2757000.024.12434814-15
AGAGTAC2223300.023.62807318-19
AACGCAG2728600.023.14731414-15
TATCAAC2366450.022.4557510-11
GCAGTGG5033950.022.0226633
CTACACG259700.020.0585691
CGCAGAG3075650.019.49782816-17
ACGACGC272000.019.0114965
CAGAGTA2642600.018.9512518-19
Produced by FastQC (version 0.11.5)