FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34624220
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1048900.30293823225476274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG219650.032.260665
ACCGCGT223900.031.8049836
AGACCGC227500.031.4206664
CAGACCG229100.031.3171673
CCGCGTT232450.030.6476887
CGCGTTC234400.030.367518
TTCGACG23800.027.8880126
TCAGACC271300.027.8160442
CTCAGAC302150.025.8898871
GCGTTCT280650.025.5304329
AATACGG287300.025.5054532
TACGGAG290350.025.1147734
ATACGGA292250.025.03224233
GAACGAT303750.024.11369145
CAATACG313550.023.6617931
AACGATC313200.023.5085646
AAGAACG318650.023.02290243
CGTTCTC325400.022.98032210
ACGGAGA317700.022.94325335
CCCAATA328150.022.4832129
Produced by FastQC (version 0.11.5)