FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19262094
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTATGCCGTCTTCTGCTTG2455411.2747367965289755TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG304200.057.2864348
GATCGGA299900.057.221631
CTCGTAT303650.057.1017744
TCGTATG306100.057.04888545
CGTATGC306450.056.96462246
TCTCGTA304750.056.86706543
ATCGGAA305350.056.7686652
ATGCCGT309200.056.34098449
CGTCTGA310050.056.1218616
ATCTCGT309600.055.9285842
TGCCGTC312450.055.79271350
ACACGTC313200.055.77403313
TCGGAAG311900.055.4106833
GCCGTCT315050.055.28545451
CGGAAGA315250.054.7470134
ACGTCTG318450.054.65075715
CGTCTTC319200.054.529753
CCGTCTT319800.054.46429452
CACGTCT322650.054.15876414
AATCTCG316900.054.14702641
Produced by FastQC (version 0.11.5)