FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27196129
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC799720.29405655488691057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT187450.032.5133486
CAGACCG189100.032.4662173
GACCGCG187850.032.4299625
AGACCGC190350.032.0670284
CCGCGTT194800.031.2410967
CGCGTTC195500.031.204688
TCAGACC223200.028.8278732
CTCAGAC251900.026.6131631
GCGTTCT230750.026.5772239
AATACGG245350.025.08068832
TACGGAG242900.025.0659334
ATACGGA246250.025.03638633
GAACGAT254850.024.0524545
CGTTCTC265150.024.01920710
AACGATC260500.023.72332446
CAATACG267000.023.54408331
ACGGAGA263750.023.16266335
CCCAATA273050.022.96848129
AAGAACG268050.022.90101443
TTCGACG18050.022.7171786
Produced by FastQC (version 0.11.5)