FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14233242
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC302100.21224960553611047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC71250.031.7546224
TTCGACG12450.031.749366
GACCGCG70400.031.5929765
CAGACCG72400.031.2098163
ACCGCGT73500.030.1807486
CGCGTTC73550.030.0797028
CCGCGTT75100.029.5767157
TCGACGA14650.026.175847
TCAGACC90650.025.837692
GTTCGAC15150.024.5329515
CGACGAG15050.024.5001328
CTCAGAC108000.023.6614651
CGGTATA21700.022.56501620
GCGTTCT101600.021.8623189
AATACGG97100.021.38970632
ATACGGA97450.021.31288133
TACGGAG96850.021.10984234
CGTTCTC110400.020.70749510
CAATACG102650.020.57810831
GAACGAT106000.020.11940645
Produced by FastQC (version 0.11.5)