FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20854540
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC812730.38971370262782107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG171850.033.7039073
AGACCGC171400.033.6546754
ACCGCGT173250.032.980056
GACCGCG173550.032.944935
CGCGTTC179250.031.8099828
CCGCGTT180600.031.6047157
TCAGACC197100.031.0031662
TTCGACG21300.030.8830476
CTCAGAC222200.028.4653991
GCGTTCT209200.027.3828269
GTTCGAC26400.025.0324635
TCGACGA27400.024.9761857
CGTTCTC239100.024.97017910
CGACGAG27850.023.1958168
AATACGG247100.023.13606532
CGGTATA37650.022.95706420
ATACGGA246000.022.93901633
TACGGAG243300.022.92673734
CCTCTCA266700.022.53030619
CCCAATA268350.021.84298529
Produced by FastQC (version 0.11.5)