FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21681136
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTATGCCGTCTTCTGCTTG622320.28703293037781785TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG83200.052.5423448
CTCGTAT85050.051.60753644
CGTATGC85650.051.41821346
TCTCGTA85450.051.12431743
GATCGGA86000.050.7176551
TCGTATG87800.050.3270945
ATGCCGT89500.049.04123749
ATCGGAA90100.048.9757772
ATCTCGT90100.048.19062442
TGCCGTC92400.047.46948650
GCCGTCT94850.046.52322451
ACACGTC95000.046.20381513
CGTCTGA95400.045.6372616
CGTCTTC97300.045.2917753
TCGGAAG97300.045.260723
CCGTCTT99100.044.3500552
CGGAAGA99250.044.135244
ACGTCTG102700.042.56565515
AATCTCG104800.041.515641
GTATGCC108900.040.60301247
Produced by FastQC (version 0.11.5)