FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21268824
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCCGTCTTCTGCTTG855550.4022554326463936TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA100100.054.0139471
TATGCCG99300.053.7335648
TCTCGTA99150.053.63699343
GCGTTAA99300.053.61664236
CTCGTAT99250.053.55323444
AGCGTTA101050.052.71728535
CGTATGC101250.052.64042746
TCGTATG103050.051.8926845
ATCGGAA104450.051.791512
CGTTAAT103000.051.71875437
ATGCCGT103450.051.60637749
ACCAGCG103700.051.45630332
ATCTCGT103700.051.3973642
TCGGAAG105300.051.239323
GCCGTCT104400.051.16502851
TGCCGTC104600.051.095450
ACACGTC105950.050.83409513
CGTCTGA107500.049.90907716
CGGAAGA108950.049.522724
CAGCGTT108450.049.14805634
Produced by FastQC (version 0.11.5)