FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13743747
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC367690.2675325731767326No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT174780.12717055981894895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18950.033.9343266
GACCGCG82400.032.9398885
ACCGCGT83100.032.834676
AGACCGC85000.032.3531764
CAGACCG85700.032.2954983
CGCGTTC87500.031.2506398
CCGCGTT88350.030.9832577
GTTCGAC21500.030.0515695
TCGACGA21750.029.9722027
TCAGACC103800.028.0570072
CGGTATA29650.027.4598720
CGACGAG23800.026.7709648
CTCAGAC120350.025.8629531
GCGTTCT108950.025.2604839
AGTTCGA28250.023.7097744
CGTTCTC122350.023.31362210
CTACACG71400.022.2264357
CAGTTCG32350.021.7993533
TACACGT79650.019.92425758
ACACGTT80350.019.8874459
Produced by FastQC (version 0.11.5)