FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27692924
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC848570.3064212359807148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC180800.032.469764
CAGACCG183800.032.164493
GACCGCG186750.031.308885
ACCGCGT191300.030.6880196
CCGCGTT192850.030.5475657
CGCGTTC193400.030.3996818
TCAGACC219700.028.1306152
AATACGG220850.026.58091532
ATACGGA222550.026.45740333
TACGGAG221750.026.35359234
CTCAGAC246450.026.136611
GCGTTCT230350.025.689819
GAACGAT234950.025.32504545
AACGATC239800.025.04662146
CAATACG241300.024.47490731
AAGAACG248350.024.0061643
ACGGAGA246650.023.81272735
AGAACGA253300.023.51373744
CCCAATA254350.023.38140929
ACGATCA258650.023.20985647
Produced by FastQC (version 0.11.5)