FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14226627
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC339960.238960366360909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG80650.032.3720055
AGACCGC82100.032.0524754
ACCGCGT82800.031.5655046
CAGACCG85250.031.0065523
TTCGACG14250.030.0166686
CCGCGTT89900.029.0724647
CGCGTTC91900.028.4718658
TCAGACC103100.026.8400962
GTTCGAC16050.025.181195
CTCAGAC116700.025.0177231
CGACGAG16450.024.9262358
GCGTTCT111500.023.5720259
TCGACGA18200.023.1778137
CGGTATA23050.022.65250620
CGTTCTC126000.021.63201710
CTACACG53050.020.96357357
AGTTCGA23200.018.94694
TACACGT61700.018.02440558
CCTCTCA154650.017.58718919
CAGTTCG28050.017.5640013
Produced by FastQC (version 0.11.5)