FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22441274
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGCCGTCTTCTGCTTG3391661.5113491328522612TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGCCGTCTTCTGCTTGA588340.26216871644631223TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA370100.057.4885331
TATGCCG442500.047.39008348
CGTATGC443850.047.2991146
CTCGTAT441500.047.25021444
ATCGGAA451800.047.1179662
TCTCGTA443700.047.11565843
TCGTATG446600.047.00125545
CGTCTGA449600.046.96319216
ATGCCGT446800.046.9472149
ACACGTC451000.046.92860413
GCCGTCT448350.046.87701451
TGCCGTC447800.046.8028450
TCGGAAG455900.046.7189873
ATCTCGT448400.046.64154442
CGGAAGA457950.046.475774
CATCTCG451950.046.26212741
CGTCTTC453850.046.2569453
ACGTCTG456800.046.24234415
CCGTCTT456100.046.10635852
CACGTCT461850.045.83890514
Produced by FastQC (version 0.11.5)