FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22725322
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25600.038.7012061
TATGCCG26700.036.56876448
CGACCAT26800.036.2110637
CTCGTAT27550.035.54648244
CGTATGC28600.033.9320446
ACACGAC28700.033.9165934
ACGACCA29250.033.58137536
TCTCGTA28900.033.2736143
TGCCGTC29950.032.4036950
ATGCCGT30350.031.97662449
GCCGTCT30550.031.86384251
CACGACC30950.031.64153535
ATCGGAA31650.030.9518012
TCGTATG32350.030.4545645
ATCTCGT32950.029.8104842
CACACGA35200.028.40783533
CGTCTGA35050.028.19271716
TCGGAAG35600.027.844363
ACACGTC35950.027.56963313
CATCTCG36300.026.81559641
Produced by FastQC (version 0.11.5)