FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21204166
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1010360.4764912706304978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22450.034.1599736
AGACCGC212550.033.121884
CAGACCG214650.032.839343
GACCGCG215650.032.3718725
ACCGCGT216250.032.33976
CGCGTTC217650.032.1175238
CCGCGTT220450.031.723047
TCAGACC240450.030.7160172
CTCAGAC264750.029.0204011
GCGTTCT251600.027.9126539
AATACGG253950.027.8758232
TACGGAG251750.027.81512534
ATACGGA255350.027.7113733
CGGTATA38800.027.51962520
GTTCGAC28500.026.5255835
CGTTCTC278200.026.29378110
GAACGAT272300.026.12679345
CAATACG273700.026.11212531
ACGGAGA272000.025.7872235
AACGATC278400.025.71325146
Produced by FastQC (version 0.11.5)